na 1 49 apo tirf Search Results


apo tirf 100 × na 1 49 oil lens  (Nikon)
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Nikon apo tirf 100 × na 1 49 oil lens
Apo Tirf 100 × Na 1 49 Oil Lens, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nikon cfi  (Nikon)
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nikon apo tirf 100  (Oxford Instruments)
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plane z step z range 2d nikon na 1 49 tirf oil  (Nikon)
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eclipse te2000 e microscope  (Nikon)
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custom inverted microscope  (Nikon)
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Nikon custom inverted microscope
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motorized tirf slider  (Carl Zeiss)
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oil-immersion objective  (Carl Zeiss)
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100× objective (na 1.45 alpha-plan-fluar)  (Carl Zeiss)
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alphaplan, 100x, na 1.45  (Carl Zeiss)
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Carl Zeiss alphaplan, 100x, na 1.45
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100× tirf oil dic lens na 1.46  (Carl Zeiss)
90
Carl Zeiss 100× tirf oil dic lens na 1.46
Pore formation by LLO represses GRAF1 assembly but does not influence the number of caveolin1- or SNX9-positive structures. (A) Graphs showing the number of endocytic spots detected by live-cell <t>TIRF</t> microscopy of HeLa Flp-In TRex cells expressing different endocytic proteins treated with LLO. The number of endocytic structures present in the TIRF field was quantified every 3 s for 5 min before and after LLO addition and plotted as the relative number of spots where the first frame of respective movie was set as 1. Error bars represent the s.d. Only movies with cells showing ANXA6-mCherry recruitment to the plasma membrane after addition of LLO were quantified. Time 0 s represent the acquisition start time in all subfigures. The data represent quantifications derived from several cells and independent movies from three independent experiments n =3 except for GRAF1, where n =1 on 7 cells. (B) Representative micrographs of maximum-projected confocal z-stacks showing fixed Flp-In TRex cells expressing GFP-GRAF1. Vehicle DMSO (control), 10 μM Ionomycin and 50 μM Bapta-AM was added to the cells for 30 min followed fixation and imaging, respectively, as indicated. Scale bars 10 μm. (C) Bar plot of the number of GFP-GRAF1 structures quantified after 30 min of drug treatment. P -values from one-way ANOVA: control versus Ionomycin P <0.01, control vs Bapta AM P <0.0001, Ionomycin vs Bapta AM P <0.0001. n =3. Error bars represent s.d.
100× Tirf Oil Dic Lens Na 1.46, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Pore formation by LLO represses GRAF1 assembly but does not influence the number of caveolin1- or SNX9-positive structures. (A) Graphs showing the number of endocytic spots detected by live-cell TIRF microscopy of HeLa Flp-In TRex cells expressing different endocytic proteins treated with LLO. The number of endocytic structures present in the TIRF field was quantified every 3 s for 5 min before and after LLO addition and plotted as the relative number of spots where the first frame of respective movie was set as 1. Error bars represent the s.d. Only movies with cells showing ANXA6-mCherry recruitment to the plasma membrane after addition of LLO were quantified. Time 0 s represent the acquisition start time in all subfigures. The data represent quantifications derived from several cells and independent movies from three independent experiments n =3 except for GRAF1, where n =1 on 7 cells. (B) Representative micrographs of maximum-projected confocal z-stacks showing fixed Flp-In TRex cells expressing GFP-GRAF1. Vehicle DMSO (control), 10 μM Ionomycin and 50 μM Bapta-AM was added to the cells for 30 min followed fixation and imaging, respectively, as indicated. Scale bars 10 μm. (C) Bar plot of the number of GFP-GRAF1 structures quantified after 30 min of drug treatment. P -values from one-way ANOVA: control versus Ionomycin P <0.01, control vs Bapta AM P <0.0001, Ionomycin vs Bapta AM P <0.0001. n =3. Error bars represent s.d.

Journal: Biology Open

Article Title: Plasma membrane damage caused by listeriolysin O is not repaired through endocytosis of the membrane pore

doi: 10.1242/bio.035287

Figure Lengend Snippet: Pore formation by LLO represses GRAF1 assembly but does not influence the number of caveolin1- or SNX9-positive structures. (A) Graphs showing the number of endocytic spots detected by live-cell TIRF microscopy of HeLa Flp-In TRex cells expressing different endocytic proteins treated with LLO. The number of endocytic structures present in the TIRF field was quantified every 3 s for 5 min before and after LLO addition and plotted as the relative number of spots where the first frame of respective movie was set as 1. Error bars represent the s.d. Only movies with cells showing ANXA6-mCherry recruitment to the plasma membrane after addition of LLO were quantified. Time 0 s represent the acquisition start time in all subfigures. The data represent quantifications derived from several cells and independent movies from three independent experiments n =3 except for GRAF1, where n =1 on 7 cells. (B) Representative micrographs of maximum-projected confocal z-stacks showing fixed Flp-In TRex cells expressing GFP-GRAF1. Vehicle DMSO (control), 10 μM Ionomycin and 50 μM Bapta-AM was added to the cells for 30 min followed fixation and imaging, respectively, as indicated. Scale bars 10 μm. (C) Bar plot of the number of GFP-GRAF1 structures quantified after 30 min of drug treatment. P -values from one-way ANOVA: control versus Ionomycin P <0.01, control vs Bapta AM P <0.0001, Ionomycin vs Bapta AM P <0.0001. n =3. Error bars represent s.d.

Article Snippet: Spinning disc confocal live-cell microscopy movies were acquired using a 63× objective lens (Plan-Apochromat 1.40 Oil DIC M27) in a Cell Observer Spinning Disc Confocal Microscope system (ANDOR iXon Ultra) (Zeiss) and TIRF microscopy using a 100× TIRF oil (NA 1.46) DIC lens controlled by the ZEN Software.

Techniques: Microscopy, Expressing, Clinical Proteomics, Membrane, Derivative Assay, Control, Imaging